Transcriptome profiling combined with network analysis deepens the understanding of immune response mechanisms in blood of pacific oyster Crassostrea gigas infected by Vibrio alginolyticus
نویسندگان
چکیده
Mass mortalities of oysters during the summer have been reported for years. Among biological factors causing oyster death in summer, Vibrio infection is considered to be most important factor. alginolyticus one main pathogens diseases. V. can cause disease and fish, shrimp, shellfish, other aquatic products. Human with also skin infections, external ear canal Hemocytes blood Crassostrea gigas resemble vertebrate macrophages play an role immune regulation organisms. Therefore, it great significance analyze transcriptome study anti-pathogen mechanism C. . In our study, we performed a profile gene expression on infected at 12 48 hours, identified 2,494 1,165 differentially expressed genes (DEGs) two sampling time points. Then, functions DEGs were using GO (Gene Ontology) KEGG (Kyoto Encyclopedia Genes Genomes) functional enrichment analysis, revealing that there multiple significantly enriched terms signaling pathways related immunity. And protein-protein interaction (PPI) network was construct significant immune-related pathways. At last, screened validated key protein numbers or involved more pathways, verified their changes by quantitative RT-PCR (qRT-PCR). For first time, studied defense based network, which explains how lacking specific immunity survive pathogen-rich environment. This research provides reference solving problem high mortality mollusks future production some disease-resistant
منابع مشابه
Whole Transcriptome Profiling of Successful Immune Response to Vibrio Infections in the Oyster Crassostrea gigas by Digital Gene Expression Analysis
The cultivated Pacific oyster Crassostrea gigas has suffered for decades large scale summer mortality phenomenon resulting from the interaction between the environment parameters, the oyster physiological and/or genetic status and the presence of pathogenic microorganisms including Vibrio species. To obtain a general picture of the molecular mechanisms implicated in C. gigas immune responsivene...
متن کاملanalysis of power in the network society
اندیشمندان و صاحب نظران علوم اجتماعی بر این باورند که مرحله تازه ای در تاریخ جوامع بشری اغاز شده است. ویژگیهای این جامعه نو را می توان پدیده هایی از جمله اقتصاد اطلاعاتی جهانی ، هندسه متغیر شبکه ای، فرهنگ مجاز واقعی ، توسعه حیرت انگیز فناوری های دیجیتال، خدمات پیوسته و نیز فشردگی زمان و مکان برشمرد. از سوی دیگر قدرت به عنوان موضوع اصلی علم سیاست جایگاه مهمی در روابط انسانی دارد، قدرت و بازتولید...
15 صفحه اولTranscriptomic response of the Pacific oyster Crassostrea gigas to hypoxia.
Marine intertidal organisms commonly face hypoxic stress during low tide emersion; moreover, eutrophic conditions and sediment nearness could lead to hypoxic phenomena; it is indeed important to understand the molecular processes involved in the response to hypoxia. In this study the molecular response of the Pacific oyster Crassostrea gigas to prolonged hypoxia (2mg O(2) L(-1) for 20d) was inv...
متن کاملThe Effects of 4-nonylphenol on the Immune Response of the Pacific Oyster, Crassostrea Gigas, following Bacterial Infection (vibrio Campbellii)
متن کامل
Functional characterisation of phagocytes in the Pacific oyster Crassostrea gigas
Invertebrates lack canonical adaptive immunity and mainly rely on innate immune system to fight against pathogens. The phagocytes, which could engulf and kill microbial pathogens, are likely to be of great importance and have to undertake significant roles in invertebrate immune defense. In the present study, flow cytometry combined with histological and lectin staining was employed to characte...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Frontiers in Marine Science
سال: 2022
ISSN: ['2296-7745']
DOI: https://doi.org/10.3389/fmars.2022.1017445